Isolate total RNA with effective genomic DNA elimination from cultured cells & tissues using mini spin columns
The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.|
|Starting Material||Cultured cells and tissues|
|Starting Amount||1 x 107 eukaryotic cells or 25-30 mg tissue|
|Elution Volume||40-70 μL|
|RNA Binding Technology||Silica Mini spin column|
|Processing Mode||Manual (centrifugation or vacuum)|
|Throughput||1 – 24|
|Final Purified Product||Total RNA (> 200 nt)|
|RNA Yield||Up to 100 µg|
|Processing Time||25 min|
|Special Notes||RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step|
Figure 1. RNA yields obtained using the Omega and Company Q’s kits with tissue samples and cultured cell samples. One-way ANOVA followed by Tukey’s post-hoc analysis indicates that the RNA yield extracted using Omega Bio-tek’s kit was significantly better than that of Company Q’s (p < 0.01) for both the sample types tested.
Figure 2. Purified RNA from tissue samples was analyzed on an Agilent Tapestation 2200 to provide integrity along with gel images. a) representative electropherogram – Company Q; b) representative electropherogram – Omega Bio-tek.
Figure 3. Purified RNA from cultured cells was analyzed on an Agilent Tapestation 2200 to provide integrity along with gel images. a) Representative electropherogram – Company Q; b) representative electropherogram – Omega Bio-tek.
Figure 4. Genomic DNA contamination was estimated in the RNA purified using kits from Omega Bio-tek and Company Q.